GsMTx4 ke 35-amino acid peptide e nang le dibond tse nne tsa disulfide tse etsang cysteine knot motif, e leng tšobotsi e tloaelehileng ea li-peptide tse ngata tsa mahloko a sekho tse fanang ka botsitso le ho khetheha.Mokhoa oa ts'ebetso oa GsMTx4 ha o hlakisoe ka botlalo, empa ho lumeloa hore o tlamella libaka tsa extracellular kapa transmembrane tsa cationic MSCs le ho thibela pore ea bona ea ho bula kapa ea ho koala ka ho fetola conformation ea bona kapa tsitsipano ea membrane.GsMTx4 e bonts'itsoe ho thibela li-MSC tse 'maloa tsa cationic ka khetho le matla a fapaneng.Mohlala, GsMTx4 e thibela TRPC1 ka IC50 ea 0.5 μM, TRPC6 e nang le IC50 ea 0.2 μM, Piezo1 e nang le IC50 ea 0.8 μM, Piezo2 e nang le IC50 ea 0.3 μM, empa ha e na phello ho TRPV41 ho TRPV11 μM.(Bae C et al 2011, Biochemistry)
GsMTx4 e 'nile ea sebelisoa e le sesebelisoa sa meriana ho ithuta mosebetsi le taolo ea cationic MSCs mefuteng e fapaneng ea lisele le lisele.E meng ea mehlala ke:
GsMTx4 e ka thibela li-MSC tse sebelisoang ka ho otlolla li-astrocyte, lisele tsa pelo, lisele tse boreleli tsa mesifa le lisele tsa mesifa ea masapo.Li-astrocyte ke lisele tse nang le sebōpeho sa linaleli tse tšehetsang boko le lesapo la mokokotlo.Lisele tsa pelo ke lisele tse etsang mesifa ea pelo.Lisele tsa mesifa tse boreleli ke lisele tse laolang motsamao oa litho tse kang mpa le methapo ea mali.Lisele tsa mesifa ea masapo ke lisele tse nolofalletsang ho tsamaea ha 'mele ka boithatelo.Ka ho thibela li-MSC ka liseleng tsena, GsMTx4 e ka fetola thepa ea bona ea motlakase, maemo a khalsiamo, ho nyenyefala le ho phomola, le ho hlahisa liphatsa tsa lefutso.Liphetoho tsena li ka ama tsela eo lisele tsena li sebetsang ka eona ka tloaelo kapa maemong a mafu (Suchyna et al., Nature 2004; Bae et al., Biochemistry 2011; Ranade et al., Neuron 2015; Xiao et al., Nature Chemical Biology 2011)
GsMTx4 e ka boela ea thibela mofuta o khethehileng oa MSC o bitsoang TCAN, o amehang karabelong ea bohloko.TACAN ke mocha o hlahang liseleng tsa methapo tse utloang bohloko.TACAN e ts'oaroa ke ts'usumetso ea mochini, joalo ka khatello kapa ho penya, 'me e baka maikutlo a bohloko.GsMTx4 e ka fokotsa ts'ebetso ea TCAN le ho fokotsa boits'oaro ba bohloko mefuteng ea liphoofolo tsa bohloko ba mochini (Wetzel et al., Nature Neuroscience 2007; Eijkelkamp et al., Nature Communications 2013)
GsMTx4 e ka sireletsa li-astrocyte ho chefo e bakoang ke molek'hule e bitsoang lysophosphatidylcholine (LPC), e leng moemeli oa lipid o bakang tšenyo bokong le mokokotlong.LPC e ka kenya tšebetsong li-MSC ho li-astrocyte mme ea etsa hore li nke khalsiamo e ngata haholo, e lebisang khatellong ea oxidative le lefu la lisele.GsMTx4 e ka thibela LPC ho kenya li-MSC ho li-astrocyte le ho li sireletsa ho chefo.GsMTx4 e ka boela ea fokotsa tšenyo ea boko le ho ntlafatsa ts'ebetso ea methapo ho litoeba tse kentsoeng ka LPC (Gottlieb et al., Journal of Biological Chemistry 2008; Zhang et al., Journal of Neurochemistry 2019)
GsMTx4 e ka fetola phapang ea lisele tsa neural stem ka ho thibela mofuta o itseng oa MSC o bitsoang Piezo1, o hlahisoang ka lisele tsa neural stem.Lisele tsa Neural stem ke lisele tse ka etsang li-neurone tse ncha kapa mefuta e meng ea lisele tsa boko.Piezo1 ke mocha o sebelisoang ke mekhoa ea mechine e tsoang tikolohong, e kang ho satalla kapa khatello, 'me e susumetsa hore na lisele tsa neural stem li etsa qeto ea hore na sele e tla ba mofuta ofe.GsMTx4 e ka kena-kenana le ts'ebetso ea Piezo1 mme ea fetola phapang ea lisele tsa neural ho tloha ho li-neuron ho ea ho li-astrocyte (Pathak et al., Journal of Cell Science 2014; Lou et al., Cell Reports 2016)
Re baetsi ba polypeptide Chaena, ba nang le phihlelo ea lilemo tse 'maloa tlhahiso ea polypeptide.Hangzhou Taijia Biotech Co., Ltd. ke moetsi oa thepa e tala ea polypeptide, e ka fanang ka mashome a likete a lisebelisoa tse tala tsa polypeptide hape e ka etsoa ho latela litlhoko.Boleng ba lihlahisoa tsa polypeptide bo botle haholo, 'me bohloeki bo ka fihla ho 98%, e' nileng ea hlokomeloa ke basebelisi lefatšeng lohle.
