Pepstatin ke pentapeptide, inhibitor ea tlhaho ea aspartyl protease, e ka thibelang aspartic protease le acid protease ea likokoana-hloko tse fapaneng.Pepstatin e hlahisoa haholo ke mefuta ea Streptomyces mme e hlahisoa ke Streptomyces.E ka thibela pepsin, pepsin D le enzyme e lokollang angiotensin, 'me e na le litlamorao tsa kalafo ho seso sa ka mpeng, khatello ea mali ea renal, ramatiki, carrageenan edema le mafu a mang.
Pepstatin ke inhibitor e matla ea aspartyl proteases tse kang pepsin, cathepsin D, le renin.Pentapeptide ena ea tlhaho e arohaneng le actinomycetes e bile ka lilemo tse ngata renin inhibitor in vitro.Pepstatin ha e tobane le renin mme ha e qhibilihe hantle metsing.Pepstatin e na le γ e sa tloaelehang ea amino acid e ka nkang sebaka sa li-amino acid tse peli ho bond ea scissile ea substrate ea protheine le ho thibela ho petsoha ha substrate ka lebaka la papiso ea sebopeho. ho boemo ba phetoho ea peptide bond hydrolysis ka aspartyl proteases.
Ha protheine e ntšoa liseleng tse robehileng, liprotheine li ka lokolloa, tse hlokang ho thibeloa ka potlako ho thibela protheine hore e se ke ea senyeha.Ka ts'ebetsong ea ho ntša liprotheine, li-protease inhibitors li hloka ho kenngoa ho thibela proteolysis.Protease inhibitor ka kakaretso e bolela ntho e kopanyang lihlopha tse ling setsing se sebetsang sa limolek'hule tsa protease, e le hore tšebetso ea protease e fokotsehe kapa e nyamele, empa ha e fetole protheine ea enzyme.Kutloisiso ea li-protease tse fapaneng ho liprotheine tse fapaneng li fapane, ka hona hoa hlokahala ho lokisa bongata ba li-protease tse fapaneng.Hobane ho qhibiliha ha protease inhibitor ka har'a mokelikeli ho le tlase haholo, ho lokela ho lebisoa tlhokomelo e khethehileng hore protease inhibitor e lokela ho tsoakoa ka ho feletseng ka har'a buffer ho fokotsa ho na ha protease inhibitor.Pepstantin A e ka thibela liprotheine tsa acid tse kang pepsin, angiotensin, collagenase, cathepsin D le chymosin.
Pepstatin A ke inhibitor ea cathepsin d le e.Ka mor'a hore lisele tsa HEK293 li phekoloe ka mefuta e fapaneng ea pepstatin A bakeng sa lihora tse 24, polelo ea LC3Ⅱ le p62 e ile ea fumanoa.Liphetho li bontšitse hore pepstatin A e ka ntlafatsa haholo polelo ea LC3Ⅱ le P62 (P<0.05) ka mokhoa o itšetlehileng ka tekanyo.20μ g/ml pepstatin A e ne e sebelisetsoa ho phekola HEK293 ka linako tse fapaneng, 'me liphello tsa linako tse fapaneng tsa nako polelong ea LC3II le p62 li ile tsa hlokomeloa.Liphetho li bontšitse hore pepstatin A e ka khona ho laola polelo ea LC3II le p62 ka mokhoa o itšetlehileng ka nako.
Re baetsi ba polypeptide Chaena, ba nang le phihlelo ea lilemo tse 'maloa tlhahiso ea polypeptide.Hangzhou Taijia Biotech Co., Ltd. ke moetsi oa thepa e tala ea polypeptide, e ka fanang ka mashome a likete a lisebelisoa tse tala tsa polypeptide hape e ka etsoa ho latela litlhoko.Boleng ba lihlahisoa tsa polypeptide bo botle haholo, 'me bohloeki bo ka fihla ho 98%, e' nileng ea hlokomeloa ke basebelisi lefatšeng lohle.
